| SpCrossreactivity | Recommended usage: 50 mM Tricine buffer, pH 7.5, containing 100 mM NaCl, 10 mM CaCl2, and 1 mMZnCl2, 10 ¿M TACE Substrate II, 600 nM TACE; incubate at 27°C with mechanical shaking; reaction can be quenched with 50 mM EDTA (final concentration) ; cleavage monitored by excitation at ~ 320 nm and emission at ~ 395 nm |