| SpCrossreactivity | Mouse Ang-3 (1-509 aa, Davis et al 1996)) was expressed as a fusion protein containing a his-tag at the C-terminus in mouse NSO cells and purified (>95%). Recombinant protein calculated mol wt is ~58 kDa but the protein migrates as 3 bands ~85-90 kDa band (reducing SDS-PAGE) due to glycosylation. |