What are Protein Blots

Studies on distribution of a given protein in various tissues are carried out to find its probable function. In addition, the protein levels may vary according to the age or physiological or pathological conditions. There is also evidence of selective processing of mRNA (alternative splicing, posttranslational modifications (glycosylation, phosphorylation, etc) or interaction with other modifying partners. Therefore, mRNA and protein levels may not always correlate. It is important to study the actual levels of a given protein in various tissues. However, acquisition of animals, preparation of appropriate protein extracts, and subsequent processing of protein gels is not only time-consuming and expensive, but also requires expertise and training in tissue processing and preparation of protein samples.

In order to simplify and expedite this process, ADI has carefully dissected and processed several major tissues from rat and mouse. Total tissue proteins have been, extracted, electrophoresed, electro-blotted, and blocked. A lane of pre-stained mol. wt markers is included in each blot to assist you in identifying the size of the proteins. These blots can be part of an initial feasibility study to see if there is an interesting aspect related to a protein distribution. A more detailed, controlled study can then be initiated to elaborate initial findings.