Pre-activated agarose to couple peptides and proteins

Antibodies to small peptide or haptens are generally raised by coupling to large carrier proteins such as Keyhole limpet Hemocyanin (KLH), Bovine serum albumin (BSA), Ovalbumin and thyroglobulin etc. However, antibodies are produced to the carrier proteins (KLH, BSA etc) and the small peptide/hapten. Many recombinant proteins are expressed and purified from E. coli. Often, it is difficult to remove the contaminating E. coli proteins. Antibodies made to proteins containing the E. coli protein contaminants can be removed by immunoadsorption over the E. coli protein-agarose column. Similarly, antibodies made to GST-fusion proteins can be immunoadsorbed over the GST-Agarose to remove anti-GST antibodies.