KitContents | 1. 1 vial of Preactivated HRP (2 mg; lyophilized) # 80221
2. Conjugation Buffer, 0.1 M NaHCO3, pH 9.5 (10 X), 100 ml; (dilute 1:10 with water before use) # 80222
3. Blocking Buffer; 500 ul; # 80223
4. Conjugate Stabilizer; 2 ml., # 80224
5. Dialysis bag (Cut off size 10-12 kda) # 80225
6. If proteins of 10kda or less are being coupled then it is a good idea to use membranes with lower cut-off such as (3 kda). Wash the membrane thoroughly with 70% ethanol and then with distilled water. |
MSDS | TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates |
Product Type | Kit |
Samples | user supplied proteins, peptides or antibodies |
SpCrossreactivity | General purpose reagents for coupling antibodies or proteins to HRP. |