| Form | Stds= 0-5000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 150 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests |
| KitContents | 1. Purified ssDNA Coated strip plate (8 wells x 12 strips) 5311
2. Mouse Anti-ssDNA Ig?s std A (0 ug/ml) 0.65 ml 5312
3. Mouse Anti-ssDNA Ig?s std B (0.31 ug/ml) 0.65 ml 5313
4. Mouse Anti-ssDNA Ig?s std C (0.62 ug/ml) 0.65 ml 5314
5. Mouse Anti-ssDNA Ig?s std D (1.25 ug/ml) 0.65 ml 5315
6. Mouse Anti-ssDNA Ig?s std E (2.5 ug/ml) 0.65 ml 5316
7. Mouse Anti-ssDNA Ig?s std F (5 ug/ml) 0.65 ml 5317
8. Gaot Anti-mouse IgG HRP conjugate (H+L),
9. 0.120 ml (100X) 5318
10. Sample Diluent (10X), 10ml SD-10
11. Wash Solution (100X), 10 ml WB-100
12. TMB Substrate solution, 12 ml 80091
13. Stop solution, 12 ml 80101
14. Complete Instruction Manual. M-5310 |
| MSDS | TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates |
| Product Type | ELISA Kit |
| Samples | Serum. Plasma, Culture medium and other fluids may be adapted |
| SpCrossreactivity | Serum samples known to be positive for anti-extractable nuclear antibodies (ENA), anti-dsDNA, anti-rheumatoid factor (RF), anti-toxoplasma gondii IgG, IgM, and anti-Cytomegalovirus IgG were tested in anti-DNA ELISA kit were found to be negative, confirming the specificity of this assay. The S1-nuclease treatment of coated plates does not affect the binding of anti-DNA IgG indicating virtual absence of ssDNA on the coated surface. The antibody conjugate used in the kit is anti-mouse IgG (H+L) that will detect most Ig?s (IgG+A+M) antibodies to DNA. ADI has developed other kits for the measure of anti-ssDNA IgG or IgM isotypes. Anti-mouse ssDNA IgG kit has not been tested in other species. A separate kit for human anti-ssDNA antibody is available. |
| SpeciesReactivity | Mouse |
