| Antigen | Recombinant human Tie-1 protein (22-760 aa, EC domain) was expressed as His-tagged Fc protein Chimera and purified (>90%). Recombinant chimeric Tie-1 protein migrates as ~130-140 kda under reducing conditions. For Western blot +ve control (Cat # TIE12-C) is supplied in SDS-PAGE sample buffer (reduced |