| Antigen | Recombinant Mouse Tie-2 protein (23-744 aa, EC domain) was expressed as His-tagged Fc protein Chimera (~107 kda) and purified (>90%). For Western blot +ve control (Cat # TIE24-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of TIE24-C for good visibility with antibody Cat # TIE24-A or other anti-mouse Tie-2 antibodies |