| Antigen | Smooth muscle actin has been purified (mol wt ~43 kDa, >95%) from chicken gizzard. Purified preparation is predominantly gamma isoform (80%) and 20% cytoplasmic actin. It is supplied in stabilizing buffer (5 mM Tris-HCI pH 8, 0.2 mM CaCl2, 0.2 mM ATP and 0.5 mM DTT) as liquid at 1 mg/ml or lyophilized in the buffer. The protein is biologically active using actin polymerization assay. |