| Antigen | 14-aa peptide from Rat ABCG8 (protein accession # P58428, refs 1); Designation (ABCG82-P, control peptide /blocking peptide) |
| Form | Antigen/blocking Peptide |
| Product Type | Peptide |
| SpCrossreactivity | Rat ABCG82-P control peptide is 100% conserved in rat ABCG8 isoforms 1-3. Antibody reactivity and actual expression of various protein isoforms and their physiological relevance is not understood. It is 93% conserved in mouse, 80% in human and 73% in Chimp ABCG8. No significant conservation of ABCG82-P is observed with other ABCGs (ABCG1-7) or other proteins. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity |
| SpeciesReactivity | Rat |
| SugApplications | Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. |