| Antigen | For WB positive control (#IGFBP33-C), mouse IGFBP-3 (22-291-aa,) was expressed as human CD33 signal peptide in NSO cells and purified. Recombinant protein ~40-50 Kda.
(>95%). For Western blot +ve control (Cat # IGFBP33-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of IGFBP33-C for good visibility with antibody Cat # IGFBP33-M. |