| Antigen | For WB positive control (#IGFBP53-C), mouse IGFBP-5 (mol wt ~29 kDa) was expressed in NSO cells as CD33 fusion tag protein, and purified >95%, ~28-38 kda ). For Western blot +ve control (Cat # IGFBP53-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of IGFBP53-C for good visibility with antibody Cat # IGFBP53-M. |