| SpCrossreactivity | Mouse PREF12-P sequence is 93% conserved in human and rat, and 87% in bovine Pref-1. Since the epitope of PREF12-S antibody is located near the cytoplasmic C-terminus, this antibody will not detect the ectodomain/ soluble forms of Pref-1. Antibody cross-reactivity in various other species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity |