| Antigen | Trimeric form of adiponectin (C39A) was expressed in HEK293 cells stably transfected with pchAd-Fvector encoding FLAG epitope-tagged mouse adiponectin. Recombinant adiponectin ? Trimeric form was purified using anti-FLAG M2 affinity gel and FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution. Purity (>95%). |