| Antigen | The DNA sequence encoding the human BMP-2 signal peptide and propeptides (aa residues 1-282 of human BMP-2) fused to the human BMP-7 mature chain (aa residues 293-431 of human BMP-7) was expressed in Chinese hamster ovary cell line. The mature recombinant human BMP-7, due to glycosylation the rhBMP-7 migrates as an ~30-40 KD protein under non-reducing conditions and as 18-20 KD protein under reducing conditions in SDS-PAGE. Purified recombinant protein for Western blot +ve control is supplied in SDS-PAGE sample buffer. Load 10 ul/lane of BMP71-C (human BMP-6) for good visibility with antibody Cat # BMP71-M. |