| Antigen | The DNA sequence encoding human BMP-2 signal peptide and propeptides (aa residues 1-282 of human BMP-2) fused to the human BMP-4 mature chain (aa residues 293-408 of human BMP-4) was expressed in a mouse myeloma cell line, NSO. The mature recombinant human BMP-4, generated by the proteolytic removal of the signal peptide and propeptides, is a disulphide-linked homodimeric protein consisting of two 116 aa residue subunits with a mol. mass of ~26kD. Due to glycosylation the rhBMP-4 migrates as an ~36kD protein under non-reducing conditions and as 18kD protein under reducing conditions in SDS-PAGE |