| Antigen | The DNA sequence encoding the human BMP-2 signal peptide and propeptides (aa residues 1-282 of human BMP-2) fused to the human BMP-7 mature chain (aa residues 293-431 of human BMP-7) was expressed in Chinese hamster ovary cell line. The mature recombinant human BMP-7, generated by the proteolytic removal of the signal peptide and propeptide, is a disulfide-linked homodimeric protein consisting of two 139aa residue subunits with a mol. mass of ~15.7 KD. Due to glycosylation the rhBMP-5 migrates as an ~30-40 KD protein under non-reducing conditions and as 18-20 KD protein under reducing conditions in SDS-PAGE.
Purified recombinant human (cat # BMP75-R-5 and BMP75-R-10) are supplied in buffer 1% sucrose, 1.2% mannitol, 20mM Glycine, 005% Tween. pH 4 in powder form with no preservatives or carrier proteins. BMP-7 has endotoxin level (<0.1 ng/1 ug). |