| Antigen | The DNA sequence encoding a chimeric protein containing the human BMP-2 signal peptide and propeptides (aa residues 1-282 of human BMP-2) fused to the human BMP-5 mature chain (aa residues 323-454 of human BMP-5) was expressed in a mouse myeloma cell line, NSO. The recombinant BMP-5 is a disulphide-linked homodimeric protein consisting of two 167 aa residue subunits with a mol. mass of ~36kD protein under non-reducing conditions and as doublet of 20kD and 25kD protein under reducing conditions in SDS-PAGE. Purified recombinant protein for Western blot +ve control is supplied in SDS-PAGE sample buffer. Load 10 ul/lane of BMP51-C (human BMP-5) for good visibility with antibody Cat # BMP51M |